While carrying out this experiment, the specimen will be held into position using a stereotactic frame during the administration of the procedure. The faraday cage will be used together with electronic amplifiers for electrophysiological recordings. We will conduct three experiments on a six channel micro fabricated silicon devise connected to a Silica Catheter.
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The first experiment will be to determine effects of saline infusions administered using the probe on concurrent electrophysiology.1??l aCSF will be administered using the device direct to the brain tissue. The second experiment will be to examine damage by the probe insertion and distribution of drugs in tissue Hoechst 33342 (Invitrogen) and propidium iodide (PI) (Invitrogen) each at 1 mg/ml in the artificial cerebral spinal fluid? will be used, (Mertensel., 2014). And finally, the third experiment will be to determine the physiological relevant quantity of drug to be administered in a regulated manner determined by probing the performance and design? in 0.6% agarose gel. Comparisons will be drawn to determine backflow for infusion rates below 0.5 ??l/minvs. those above 1.0 ??l/min. The 16-channel micro fabricated silicon apparatus will be prefilled with the infusate to prevent air from being infused the brain in the process.Sincethe solution will return to room temperature, Eventually, it will be warmed to body temperature due to the small volume that will be used. The incision will be positioned above the craniotomy,and the starting trial depth will be 2mm at a speed of 1.3 mm/sec. Ifno action potential is realized, withdrawal or further insertion will be applied.This is to determine the infusion rate and the insertion speed to be applied to all specimen in the experiment.
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