Atmospheric aerosols on human health and effect

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A highly Sensitive (LOD; 0.04-0.4 ng/ml) method is developed for detection and quantification of acidic compounds (C3 -C10) containing mono and dicarboxylic acids on GC-MS. These compounds (C3 -C10) existed in trace amount, as secondary organic aerosols i.e. important constituents of Aerosols.

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Membrane extraction technique was utilized for selective enrichment (1-4300 times) of target compounds. Good repeatability (RSD% ≤ 10%) from selective organic phase (10% TOPO in DHE) was achieved with three phase HF-LPME. Aerosols containing samples, after Ultrasonic Assisted extraction were detected and quantified Through GC-MS. Effective derivatization of each target compound was performed with BSTFA reagent. Gas Chromatography, having capillary column and interfaced with mass spectrometry was used for separation, detection and quantification of target compounds.

Method Development and Application -hollow fiber Supported liquid membrane extraction of Fatty acids (C3-C10) containing mono and dicarboxylic acids and Detection of aerosols Samples after ultrasonic assisted extraction.

1. Introduction:

Impact of Atmospheric aerosols on human health and effect on radioactive stability in Earth’s atmosphere is getting importance now a days and this phenomenon has been well understood. [1]. Atmospheric aerosols can harm respiratory and cardiovascular system of human.

Impact of Secondary organic aerosols as biogenic and anthropogenic antecedent is identified (Adams and sinfold, 2002) [1, 17]. Low molecular dicarboxylic acids (C3-C9) are also vital tracers of SOA [2]. Short chain fatty acids are found as secondary organic aerosols which are also supposed to derive from long chain fatty acids [1]. Importance of organic aerosol has been well established now a days and carboxylic acids are of great interest for environmental studies [1]. Several studies and mechanisms were proposed to understand the production of these SOA precursors [1]. Short chain carboxylic acids are found extensively in troposphere [2]. Secondary organic aerosols (SOA) are formed in the atmosphere by gas particles conversions. Organic matter present in aerosol contains more than 90% of troposphere’s aerosols [5, 15].

Dicarboxylic acids found in nature as polymeric compounds such as suberin and cutin [3]. Short chain dicarboxylic acids are found in vegetables [Siddiqui, 1989] and in soil containing micro organisms of durum wheat [4]. Dicarboxylic acids are found in plant oils which have greater interest for cosmetic and pharmaceutical industries [6]. Short chain dicarboxylic acids having aliphatic chain possess strong cyclotoxicity and antineoplastic activities [18].

Many analytical techniques are used to determine the composition of SOA so keeping in view these techniques new method for determination of fatty acids (common in SOA) has been developed. Membrane extraction is used in this method due to its increasing importance for high selectivity and high enrichment factor [24].

Dicarboxylic acids formed of bio oxidation of fatty acids so these are considered as metabolic part of fatty acid [42].

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